rabbit mab phosphonf κb p65 ser536 93h1 rabbit mab  (Cell Signaling Technology Inc)

Journal: Food & function

Article Title: Characterization of different stages of Maillard reaction in soy: impact on physicochemical properties and immunogenicity of soy proteins.

doi: 10.1039/d4fo04400b

Figure Lengend Snippet: Fig. 5 Expression of phosphorylated NFkB p65 shows an increased trend in macrophages after stimulation with glycated soy. Expression of phosphorylated NFkB P65, analyzed by western blot (A) THP-1 differen- tiated into macrophages, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy for 10 minutes, data is representative of 3 replicates. (B) Primary derived adherent monocytes from one donor, stimulated with 25 μg ml−1 glycated soy or 100 ng ml−1 LPS and 10 ng ml−1 IFNy, data is representative of two replicates. Quantified data based on the adjusted volume normalized to beta actin. Data was processed and quantified using the ImageLab software from BioRad.

Article Snippet: The membrane was then washed 3×, followed by incubation with primary antibody (PhosphoNF-κB p65 (Ser536) (93H1) Rabbit mAb, Cell Signaling) overnight at 4 °C.

Techniques: Expressing, Western Blot, Derivative Assay, Software

Journal: Scientific reports

Article Title: RSL3 induces ferroptosis by activating the NF-κB signalling pathway to enhance the chemosensitivity of triple-negative breast cancer cells to paclitaxel.

doi: 10.1038/s41598-025-85774-w

Figure Lengend Snippet: Fig. 4. Combination treatment with PTX and RSL3 activates NF-κB and promotes TNBC cell death. (A) The viability of MDA-MB-231 and MDA-MB-468 cells was assayed using the CCK-8 assay after combination treatment with PTX or RSL3 and an NF-κB inhibitor for 24 h. (B) After the treatments, the expression levels of cytokines were detected by RT-qPCR. (C) After the treatments, the expression levels of cytokines were detected by Western blotting and ELISA. (D) The protein expression levels of P65, P-P65 and GPX4 were measured by Western blotting, and a grayscale map of protein expression is displayed. **P < 0.01; *P < 0.05; ****p < 0.0001; ***p < 0.001. NS: Not significant.

Article Snippet: NF-κB p65 (D14E12) rabbit monoclonal antibody (8242), phosphoNF-κB p65 (Ser536) rabbit monoclonal antibody (3033), and anti-rabbit IgG (H + L) (DyLightTM 800 4X PEG Conjugate) rabbit secondary antibody (5151) were purchased from Cell Signaling Technology (CST).

Techniques: CCK-8 Assay, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay